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KMID : 1177720150090010039
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Journal of Alternatives to Animal Experiments
2015 Volume.9 No. 1 p.39 ~ p.43
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Value of Keraskin™, Vm, a Reconstituted Human Epidermis, for Development of Skin Sensitization Alternative Method
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Park Jung-Eun
Gautam Ravi
Kim Chang-Yul
Jang Won-Hee
Seok Seung-Hyeok
Heo Yong
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Abstract
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Keraskin™-VM, reconstructed human epidermis, was previously investigated for its applicability in development of skin irritation alternative test methods. Considering its expression of various inflammatory mediators, Keraskin™-VM was further evaluated whether it could be useful for development of skin sensitization alternative test method. Keraskin™-VM was incubated 24h in the presence of various skin sensitizing or non-sensitizing test substances followed by collection of culture supernatants. Level of interleukin(IL)-18, a representative pro-inflammatory cytokine, in the supernatants was determined. All the skin sensitizing substances resulted in significantly higher IL-18 levels than the their vehicle control leading to maximum 64-fold, 28-fold, and 85-fold induction of IL-18 expression from 2,4-dintrochlorobenzene, eugenol, and citral, respectively. Among three non-sensitizing test substances (sodium lauryl sulfate, lactic acid, and glycerol), the maximum fold induction of IL-18 was 16 from 0.01% sodium lauryl sulfate. Cell viability was not substantially affected on the magnitude of IL-18 fold induction. Overall, our results suggest the potential usage of Keraskin™-VM for skin sensitization alternative test method.
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KEYWORD
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Skin sensitization, Alternative method, Reconstructed human epidermis, Interleukin-18
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